| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Clima Temperado. |
|
Data corrente: |
19/01/2024 |
|
Data da última atualização: |
19/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PEREIRA, P.; SANTANA, F. M.; DALLEGRAVE, A.; VAN DER SAND, S. T. |
|
Afiliação: |
PRISCILA PEREIRA, UNIVERSIDADE FEDERAL DE PELOTAS; FLAVIO MARTINS SANTANA, CPACT; ALEXSANDRO DALLEGRAVE, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; SUELI TERESINHA VAN DER SAND, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL. |
|
Título: |
Antifungal activity of Streptomyces spp. against Pyrenophora tritici-repentis the causal agent of tan spot in wheat. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Journal of Plant Protection Research, v. 63, n. 3, p. 331-339, 2023. |
|
ISSN: |
1899-007X |
|
DOI: |
https://doi.org/10.24425/jppr.2023.146871 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Tan spot, caused by Pyrenophora tritici-repentis (Ptr), is a worrisome destructive foliar disease of wheat-growing areas around the world. Streptomyces spp. have been investigated as biocontrol agents because they beneficially interact with host plants and produce important bioactive substances that can act in the suppression of diseases in plants. In the present study, antifungal activity and plant growth-promoting of Streptomyces spp. strains 6(4), R18(6), and their consortium, were evaluated through in vitro and greenhouse assays. The Ultra High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF MS) technique was used to analyze the crude extract of each strain. The results of the in vitro tests showed that the 6(4) metabolites caused several abnormalities in the conidial germination of Ptr. This strain also produced indole acetic acid (IAA) and siderophores. Strain R18(6) did not alter conidial germination of Ptr, and produced IAA and phosphate solubilizers. In the greenhouse, the treatment ‘seed inoculation plus foliar spray’ with streptomycetes propagules and metabolites contributed to biomass gain, with no statistical difference between the strains (p < 0.05). Treatments with 6(4) ‘seed inoculation’, ‘seed inoculation plus foliar spray’, and consortium ‘seed inoculation’ showed the lowest percentage of injured area compared to other treatments (p < 0.05). UHPLC-QTOF MS data showed that erucamide is present in the culture of 6(4), but not in the culture of R18(6). Therefore, this substance is one of those involved in Ptrhyphal abnormalities, and R18(6) use indirect mechanisms of action to control Ptr. We concluded that these Streptomyces spp. and their metabolites have a promising potential for biological control of Ptr to protect wheat plants from tan spot damage. MenosTan spot, caused by Pyrenophora tritici-repentis (Ptr), is a worrisome destructive foliar disease of wheat-growing areas around the world. Streptomyces spp. have been investigated as biocontrol agents because they beneficially interact with host plants and produce important bioactive substances that can act in the suppression of diseases in plants. In the present study, antifungal activity and plant growth-promoting of Streptomyces spp. strains 6(4), R18(6), and their consortium, were evaluated through in vitro and greenhouse assays. The Ultra High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF MS) technique was used to analyze the crude extract of each strain. The results of the in vitro tests showed that the 6(4) metabolites caused several abnormalities in the conidial germination of Ptr. This strain also produced indole acetic acid (IAA) and siderophores. Strain R18(6) did not alter conidial germination of Ptr, and produced IAA and phosphate solubilizers. In the greenhouse, the treatment ‘seed inoculation plus foliar spray’ with streptomycetes propagules and metabolites contributed to biomass gain, with no statistical difference between the strains (p < 0.05). Treatments with 6(4) ‘seed inoculation’, ‘seed inoculation plus foliar spray’, and consortium ‘seed inoculation’ showed the lowest percentage of injured area compared to other treatments (p < 0.05). UHPLC-QTOF MS data showed that erucamide is present in the culture of 6(4),... Mostrar Tudo |
|
Palavras-Chave: |
Mancha bronzeada. |
|
Thesagro: |
Doença; Doença Fúngica; Trigo. |
|
Thesaurus Nal: |
Pyrenophora tritici-repentis; Wheat. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161065/1/Artigo2023-Pereira.pdf
|
|
Marc: |
LEADER 02655naa a2200253 a 4500
001 2161065
005 2024-01-19
008 2023 bl uuuu u00u1 u #d
022 $a1899-007X
024 7 $ahttps://doi.org/10.24425/jppr.2023.146871$2DOI
100 1 $aPEREIRA, P.
245 $aAntifungal activity of Streptomyces spp. against Pyrenophora tritici-repentis the causal agent of tan spot in wheat.$h[electronic resource]
260 $c2023
520 $aTan spot, caused by Pyrenophora tritici-repentis (Ptr), is a worrisome destructive foliar disease of wheat-growing areas around the world. Streptomyces spp. have been investigated as biocontrol agents because they beneficially interact with host plants and produce important bioactive substances that can act in the suppression of diseases in plants. In the present study, antifungal activity and plant growth-promoting of Streptomyces spp. strains 6(4), R18(6), and their consortium, were evaluated through in vitro and greenhouse assays. The Ultra High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF MS) technique was used to analyze the crude extract of each strain. The results of the in vitro tests showed that the 6(4) metabolites caused several abnormalities in the conidial germination of Ptr. This strain also produced indole acetic acid (IAA) and siderophores. Strain R18(6) did not alter conidial germination of Ptr, and produced IAA and phosphate solubilizers. In the greenhouse, the treatment ‘seed inoculation plus foliar spray’ with streptomycetes propagules and metabolites contributed to biomass gain, with no statistical difference between the strains (p < 0.05). Treatments with 6(4) ‘seed inoculation’, ‘seed inoculation plus foliar spray’, and consortium ‘seed inoculation’ showed the lowest percentage of injured area compared to other treatments (p < 0.05). UHPLC-QTOF MS data showed that erucamide is present in the culture of 6(4), but not in the culture of R18(6). Therefore, this substance is one of those involved in Ptrhyphal abnormalities, and R18(6) use indirect mechanisms of action to control Ptr. We concluded that these Streptomyces spp. and their metabolites have a promising potential for biological control of Ptr to protect wheat plants from tan spot damage.
650 $aPyrenophora tritici-repentis
650 $aWheat
650 $aDoença
650 $aDoença Fúngica
650 $aTrigo
653 $aMancha bronzeada
700 1 $aSANTANA, F. M.
700 1 $aDALLEGRAVE, A.
700 1 $aVAN DER SAND, S. T.
773 $tJournal of Plant Protection Research$gv. 63, n. 3, p. 331-339, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
28/11/2014 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
O'BRIEN, A. M. P.; MÉSZÁROS, G.; UTSUNOMIYA, Y. T.; SONSTEGARD, T. S.; GARCIA, J. F.; TASSEL, C. P. V.; CARVALHEIRO, R.; SILVA, M. V. G. B.; SÖLKNER, J. |
|
Afiliação: |
ANA M. PÉREZ O'BRIEN, University of Natural Resources and Life Sciences, Vienna; GÁBOR MÉSZÁROS, University of Natural Resources and Life Sciences, Vienna; YURI T. UTSUNOMIYA, UNESP; TAD S. SONSTEGARD, ARS-USDA, USA; JOSÉ F. GARCIA, UNESP; CURTIS P. VAN TASSEL, ARS-USDA, USA; ROBERTO CARVALHEIRO, GenSys Consultores Associados; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; JOHANN SÖLKNER, University of Natural Resources and Life Sciences, Vienna. |
|
Título: |
Linkage disequilibrium levels in Bos indicus and Bos taurus cattle using medium and high density SNP chip data and different minor allele frequency distributions. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Livestock Science, v. 166, p. 121-132, 2014. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Linkage disequilibrium (LD), the observed correlation between alleles at different loci in the genome, is a determinant parameter in many applications of molecular genetics. With the wider use of genomic technologies in animal breeding and animal genetics, it is worthwhile revising and improving the current knowledge and understanding of cattle LD. This study analyzes levels of LD assessed through the r2 measurement in seven breeds of cattle from both indicine (Bos indicus) and taurine (Bos taurus) (sub)species, genotyped with a high density panel (HD) of over 777,000 single nucleotide polymorphism (SNP) markers. Average levels of LD were assessed for inter-marker distances from 0 kb up to 10 Mb. Two more SNP panels were built in silico from the original genotypes to represent a lower density SNP chip including approximately 50,000 SNPs (50K) and a panel of 249,000 SNPs chosen to comply with specific minor allele frequency (MAF) distributions. The results show higher levels of LD than previously reported, especially at short inter-marker distances of less than 20 kb, assessed through the use of the higher density panel. Negligible impact of MAF changes in the estimation of r2 was observed, supporting the use of the HD panel for LD-based methodologies even when ascertainment bias in the choice of SNPs is suspected. The 50K SNP panel indicated lower levels of LD in the first 100 kb and highly unreliable LD estimates for distances shorter than 50 kb. Overall, lower levels of LD were observed for all indicine breeds as compared to taurine breeds, with larger differences assessed in the first 100 kb, suggesting the need for use of higher SNP densities in this (sub)species when applying LD-dependent methods, as compared to most taurine breeds. MenosLinkage disequilibrium (LD), the observed correlation between alleles at different loci in the genome, is a determinant parameter in many applications of molecular genetics. With the wider use of genomic technologies in animal breeding and animal genetics, it is worthwhile revising and improving the current knowledge and understanding of cattle LD. This study analyzes levels of LD assessed through the r2 measurement in seven breeds of cattle from both indicine (Bos indicus) and taurine (Bos taurus) (sub)species, genotyped with a high density panel (HD) of over 777,000 single nucleotide polymorphism (SNP) markers. Average levels of LD were assessed for inter-marker distances from 0 kb up to 10 Mb. Two more SNP panels were built in silico from the original genotypes to represent a lower density SNP chip including approximately 50,000 SNPs (50K) and a panel of 249,000 SNPs chosen to comply with specific minor allele frequency (MAF) distributions. The results show higher levels of LD than previously reported, especially at short inter-marker distances of less than 20 kb, assessed through the use of the higher density panel. Negligible impact of MAF changes in the estimation of r2 was observed, supporting the use of the HD panel for LD-based methodologies even when ascertainment bias in the choice of SNPs is suspected. The 50K SNP panel indicated lower levels of LD in the first 100 kb and highly unreliable LD estimates for distances shorter than 50 kb. Overall, lower levels of LD... Mostrar Tudo |
|
Palavras-Chave: |
Indicine; Minor allele frequency. |
|
Thesaurus NAL: |
cattle; linkage disequilibrium; taurine. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02632naa a2200277 a 4500
001 2001105
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aO'BRIEN, A. M. P.
245 $aLinkage disequilibrium levels in Bos indicus and Bos taurus cattle using medium and high density SNP chip data and different minor allele frequency distributions.$h[electronic resource]
260 $c2014
520 $aLinkage disequilibrium (LD), the observed correlation between alleles at different loci in the genome, is a determinant parameter in many applications of molecular genetics. With the wider use of genomic technologies in animal breeding and animal genetics, it is worthwhile revising and improving the current knowledge and understanding of cattle LD. This study analyzes levels of LD assessed through the r2 measurement in seven breeds of cattle from both indicine (Bos indicus) and taurine (Bos taurus) (sub)species, genotyped with a high density panel (HD) of over 777,000 single nucleotide polymorphism (SNP) markers. Average levels of LD were assessed for inter-marker distances from 0 kb up to 10 Mb. Two more SNP panels were built in silico from the original genotypes to represent a lower density SNP chip including approximately 50,000 SNPs (50K) and a panel of 249,000 SNPs chosen to comply with specific minor allele frequency (MAF) distributions. The results show higher levels of LD than previously reported, especially at short inter-marker distances of less than 20 kb, assessed through the use of the higher density panel. Negligible impact of MAF changes in the estimation of r2 was observed, supporting the use of the HD panel for LD-based methodologies even when ascertainment bias in the choice of SNPs is suspected. The 50K SNP panel indicated lower levels of LD in the first 100 kb and highly unreliable LD estimates for distances shorter than 50 kb. Overall, lower levels of LD were observed for all indicine breeds as compared to taurine breeds, with larger differences assessed in the first 100 kb, suggesting the need for use of higher SNP densities in this (sub)species when applying LD-dependent methods, as compared to most taurine breeds.
650 $acattle
650 $alinkage disequilibrium
650 $ataurine
653 $aIndicine
653 $aMinor allele frequency
700 1 $aMÉSZÁROS, G.
700 1 $aUTSUNOMIYA, Y. T.
700 1 $aSONSTEGARD, T. S.
700 1 $aGARCIA, J. F.
700 1 $aTASSEL, C. P. V.
700 1 $aCARVALHEIRO, R.
700 1 $aSILVA, M. V. G. B.
700 1 $aSÖLKNER, J.
773 $tLivestock Science$gv. 166, p. 121-132, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
